|
Toluene biosensor: two strains of Pseudomonas fluorescens on barley roots that were exposed to the pollutant toluene. One strain was engineered to constituitvely express a red fluorescent reporter protein. A second strain, a "life history biosensor", was engineered to experience an irreversible, heritable induction of a green fluorescent reporter protein upon exposure to toluene. A large number of green and red cells on barly roots following root inoculation with a 60:1 ratio of red to green cells, indicate the P. fluorescens strain was exposed to toluene on these roots.
|
