Microbiology - 008 - Catalase Test

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The catalase test tests for the presence of catalase, an enzyme that breaks down the harmful substance hydrogen peroxide into water and oxygen. If an organism can produce catalase, it will produce bubbles of oxygen when hydrogen peroxide is added to it. First, ensure you have the organism of interest growing in fresh pure culture. Transfer a small amount from a colony directly to a clean glass slide using a toothpick or a sterile loop or needle. Add one drop of hydrogen peroxide and look for bubbles. Bubbles are a positive result for the presence of catalase. If no bubbles form, it is a negative result; this suggests that the organism does not produce catalase. Dispose of the slide in a slide disposal bin and any toothpicks in a biohazard bin.

The catalase test tests for the presence of catalase, an enzyme that breaks down the harmful substance hydrogen peroxide into water and oxygen. If an organism can produce catalase, it will produce bubbles of oxygen when hydrogen peroxide is added to it.

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